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atcc 7061 spore suspensions  (ATCC)


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    Structured Review

    ATCC atcc 7061 spore suspensions
    Atcc 7061 Spore Suspensions, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 352 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atcc+7061+spores/Bacillus+pumilus+Meyer+and+Gottheil/pmc03426692-272-13-13
    Average 95 stars, based on 352 article reviews
    atcc 7061 spore suspensions - by Bioz Stars, 2026-09
    95/100 stars

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    Activity Assay:

    Article Title: Protection of Bacillus pumilus Spores by Catalases
    Article Snippet: .. Although the initial catalase activity in ATCC 7061 spores was higher, it was less stable over time than the SAFR-032 enzyme. ..

    Article Title: Protection of Bacillus pumilus Spores by Catalases
    Article Snippet: .. Therefore, the increased catalase activity of ATCC 7061 spores that we observed may compensate for the absence of YjqC, although spores of this strain are not as resistant because of the enzymatic instability of BPUM_1305. ..

    Isolation:

    Article Title: Protection of Bacillus pumilus Spores by Catalases
    Article Snippet: .. In addition to ATCC 7061 spores, YjqC was also absent from B. pumilus BG-B79, a strain isolated from a biogas plant in Sweden ( 5 ). ..



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    7061 spore protein extracts  (ATCC)
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    SDS-PAGE analysis of proteins extracted from intact and decoated <t>B.</t> <t>pumilus</t> spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC <t>7061;</t> 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.
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    small acid soluble spore protein b  (ATCC)
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    SDS-PAGE analysis of proteins extracted from intact and decoated <t>B.</t> <t>pumilus</t> spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC <t>7061;</t> 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.
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    SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.

    Journal: Applied and Environmental Microbiology

    Article Title: Protection of Bacillus pumilus Spores by Catalases

    doi: 10.1128/AEM.01211-12

    Figure Lengend Snippet: SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.

    Article Snippet: B. pumilus SAFR-032 and ATCC 7061 spore protein extracts were analyzed by SDS-PAGE.

    Techniques: SDS Page, Liquid Chromatography with Mass Spectroscopy

    SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.

    Journal: Applied and Environmental Microbiology

    Article Title: Protection of Bacillus pumilus Spores by Catalases

    doi: 10.1128/AEM.01211-12

    Figure Lengend Snippet: SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.

    Article Snippet: B. pumilus SAFR-032 and ATCC 7061 spore protein extracts were analyzed by SDS-PAGE.

    Techniques: SDS Page

    Manganese catalases identified by SDS-PAGE and LC-MS/MS in B. pumilus spores

    Journal: Applied and Environmental Microbiology

    Article Title: Protection of Bacillus pumilus Spores by Catalases

    doi: 10.1128/AEM.01211-12

    Figure Lengend Snippet: Manganese catalases identified by SDS-PAGE and LC-MS/MS in B. pumilus spores

    Article Snippet: B. pumilus SAFR-032 and ATCC 7061 spore protein extracts were analyzed by SDS-PAGE.

    Techniques:

    Catalase specific activities detected in B. pumilus spore suspensions

    Journal: Applied and Environmental Microbiology

    Article Title: Protection of Bacillus pumilus Spores by Catalases

    doi: 10.1128/AEM.01211-12

    Figure Lengend Snippet: Catalase specific activities detected in B. pumilus spore suspensions

    Article Snippet: B. pumilus SAFR-032 and ATCC 7061 spore protein extracts were analyzed by SDS-PAGE.

    Techniques: Activity Assay